Journal: Frontiers in Oncology
Article Title: A positive feedback loop between tryptophan hydroxylase 1 and β-Catenin/ZBP-89 signaling promotes prostate cancer progression
doi: 10.3389/fonc.2022.923307
Figure Lengend Snippet: TPH1 promotes tryptophan (Trp) hydroxylation and 5-hydroxytryptamine (5-HT) production. (A) Culture of 10 5 DU145/PC-3 and TPH1-overexpressing DU145/PC-3 cells in 2 ml of culture medium for 48 h. Trp consumption was examined using a human Trp enzyme-linked immunosorbent assay (ELISA) kit. (B) 5-HT production in (A) was examined using a human 5-HT ELISA kit. (C) Relative proliferation of DU145/PC-3 cells treated with phosphate-buffered saline (PBS) or 5-HT (5 μM). (D) Relative number of migrating DU145/PC-3 cells treated with PBS or 5-HT (5 μM). (E) Quantification of 5-HT in tumor tissues from patients with M and NM prostate cancer (n = 10 per group) using ELISA. ** p < 0.01.
Article Snippet: A human 5-HT ELISA kit was purchased from CUSABIO Co. (China).
Techniques: Enzyme-linked Immunosorbent Assay, Saline